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KMID : 0374019800030040157
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Ewha Medical Journal
1980 Volume.3 No. 4 p.157 ~ p.162
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Qualitative Alteration in Hepatic Microsomal Cytochrome P-450 Apoproteins Associated with Bile Duct Ligation, and the Administration of Cholic Acid and Phenobarbital
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Abstract
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We have investigated in rat liver whether different forms of cytochrome P-450 are alterated in hepatic disorders associated with impaired drug metabolism. Total hepatic cytochrome P-450 is decreased after either bile duct ligation or the administration of estradiol. In contrast, phenobarbital administrated alone increases hepatic content of cytochrome P-450, and when administrated with estradiol the reduction in cytochrome-P-450 was prevented.
Four forms of microsomal cytochrome P-450 apoproteins, ranging in molecular weight from 50 000 to 58,000, were tentatively identified in a sodium dodecyl sulfate (SDS)-6M urea polyacrylamide gel electrophoresis system. Phenobarbital administration increased primarily band IV;50, 000 daltons). Bile duct ligation was associated. with a market redu.tion in bands II and III while bands II and III were decreased. with estradiol benzoate administration. Simultaneous administration of phenobarbital. and estradiol demonstrated a return of band I and an increase in density of bands III and IV; Simultaneous administration of cholic acid and estradiol demonstrated a return of band I and not altered in band III and W.
These studies support the hypothesis that multiple forms of cytochrome P-450,: are,
_. , Yk
present in liver microsomal membranes and that alterations ´in specific apoprotcins ~?´
may be associated with an increase or a decrease in the functional properties of cytoc-
hrome P-450.
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